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Thursday, 5 June 2025

The Bite That Hurts: Malaria Transmission Explained

 


Overview -

Malaria is an acute febrile illness caused by protozoan parasites of the genus Plasmodium. It remains one of the world’s most significant public health challenges, particularly in tropical and subtropical regions. The disease is transmitted to humans through the bite of an infective female Anopheles mosquito.


1. Causative Agents

There are five Plasmodium species known to commonly infect humans:

  • Plasmodium falciparum – the most lethal and widespread in sub-Saharan Africa
  • Plasmodium vivax – causes relapsing malaria; prevalent in Asia and Latin America
  • Plasmodium ovale – similar to P. vivax but less common; found in West Africa and the Pacific Islands
  • Plasmodium malariae – causes a more chronic infection; scattered distribution worldwide
  • Plasmodium knowlesi – zoonotic malaria from macaque monkeys; emerging in Southeast Asia

2. Life Cycle & Transmission

  1. Sporozoite Stage (Mosquito → Human):
    • An infected Anopheles mosquito injects sporozoites into the human bloodstream during a blood meal.
    • Sporozoites travel to hepatocytes (liver cells).
  2. Schizogony in Liver (Exoerythrocytic Stage):
    • In hepatocytes, sporozoites mature into schizonts, which rupture and release merozoites into the bloodstream.
    • In species like P. vivax and P. ovale, hypnozoites can remain dormant in the liver for weeks to months, causing relapses.
  3. Erythrocytic Cycle (Blood Stage):
    • Merozoites invade red blood cells (RBCs), develop from ring forms → trophozoites → schizonts → rupture, releasing more merozoites.
    • This cycle (approximately 48–72 hours, depending on species) corresponds to the characteristic fever spikes.
  4. Gametocyte Formation (Sexual Stage):
    • Some merozoites differentiate into sexual forms called gametocytes.
    • When a mosquito bites an infected person, it ingests gametocytes, which mature in the mosquito gut to form sporozoites.
  5. Sporozoite Development in Mosquito:
    • Within the mosquito, the parasite undergoes fertilization → ookinete → oocyst → release of sporozoites that migrate to the salivary glands—ready to infect the next human host.

3. Clinical Presentation

A. Incubation Period

  • P. falciparum: typically 9–14 days
  • P. vivax / P. ovale: around 12–18 days, but relapses may occur months later
  • P. malariae: roughly 18–40 days

B. Common Early Symptoms

  • Fever (classically tertian for P. vivax, P. ovale, P. falciparum, P. knowlesi; quartan for P. malariae)
  • Chills and rigors
  • Sweating episodes as fever resolves
  • Headache
  • Myalgia (muscle aches)
  • Malaise and fatigue

C. Gastrointestinal Symptoms

  • Nausea and Vomiting – often seen in first malaria attacks, particularly with high parasitemia (most common in P. falciparum).
  • Diarrhea or watery stools can occur, especially in children.

D. Respiratory and Other Features

  • Cough (nonproductive)
  • Shortness of breath (may indicate pulmonary edema in severe falciparum)
  • Abdominal pain (from splenomegaly or hepatomegaly)
  • Anemia (due to hemolysis of infected and uninfected RBCs)
  • Jaundice, especially when parasitemia is high

E. Severe Malaria

Most commonly caused by P. falciparum, but P. knowlesi can also lead to severe disease. Criteria include:

  • Cerebral malaria: altered consciousness, seizures, coma
  • Severe anemia: hemoglobin < 5 g/dL or hematocrit < 15 %
  • Acute kidney injury: oliguria/anuria, raised creatinine
  • Acute respiratory distress syndrome (ARDS)
  • Hypoglycemia (often in children or pregnant women)
  • Metabolic acidosis (lactic acidosis)
  • Hyperparasitemia (> 5 %–10 % infected RBCs)
  • Shock (circulatory collapse)

4. Diagnosis

  1. Microscopy (Gold Standard)
    • Thick smear: more sensitive for detecting low parasite densities; used to confirm presence/absence.
    • Thin smear: allows species identification and quantification (parasites per microliter).
  2. Rapid Diagnostic Tests (RDTs)
    • Detect species-specific antigens (e.g., histidine-rich protein II [HRP-II] for P. falciparum; pan-Plasmodium LDH).
    • Useful where microscopy is unavailable.
  3. Polymerase Chain Reaction (PCR)
    • High sensitivity and specificity; used for species confirmation, especially with low parasitemia.
    • More costly and time-consuming; often reserved for reference labs.
  4. Other Tests
    • Complete Blood Count (CBC): reveals anemia; thrombocytopenia is common.
    • Blood chemistries: renal function, liver enzymes, bilirubin, electrolytes (important for severe cases).

5. Treatment

A. Uncomplicated Malaria

  • Plasmodium falciparum (chloroquine-resistant regions):
    • Artemisinin-based Combination Therapies (ACTs) are first-line (e.g., artemether-lumefantrine, artesunate-amodiaquine).
    • In areas still sensitive to chloroquine (now rare): chloroquine (25 mg base/kg over 3 days).
  • Plasmodium vivax / Plasmodium ovale:
    1. Chloroquine (25 mg base/kg over 3 days), provided no resistance.
    2. After blood schizonticide to clear parasitemia, give primaquine (0.25 mg/kg daily for 14 days) to eradicate hypnozoites and prevent relapse.
      • Prior to primaquine, screen for G6PD deficiency to avoid hemolysis.
  • Plasmodium malariae:
    • Chloroquine is effective (typically given 25 mg base/kg over 3 days).
  • Plasmodium knowlesi:
    • Treatment parallels P. falciparum – ACTs or chloroquine where sensitive; monitor closely due to risk of rapid parasitemia rise.

B. Severe Malaria (Medical Emergency)

  • Intravenous (IV) Artesunate (preferred) or IV quinidine/quinine if artesunate unavailable.
  • Once the patient can tolerate oral medication and parasitemia has dropped, switch to an ACT.
  • Supportive care: manage hypoglycemia, transfuse for severe anemia, treat acute kidney injury, and monitor for cerebral complications.

6. Prevention & Control

A. Vector Control

  • Insecticide-Treated Nets (ITNs): sleeping under long-lasting insecticide-treated bed nets reduces mosquito bites at night.
  • Indoor Residual Spraying (IRS): spraying insecticide on interior walls; effective in regions with stable transmission.
  • Larval Source Management: draining or treating standing water where Anopheles mosquitoes breed.

B. Personal Protective Measures

  • Use of Insect Repellents: DEET or picaridin-based repellents on exposed skin.
  • Wearing Protective Clothing: long sleeves and pants, especially during dawn/dusk when mosquitoes are most active.

C. Chemoprophylaxis (for Travelers)

  • Atovaquone–proguanil: begin 1–2 days before travel, continue daily during stay and 7 days after leaving.
  • Doxycycline: start 1–2 days before, daily during travel, and 4 weeks after departure.
  • Mefloquine: weekly dosing beginning 2 weeks before travel, during travel, and 4 weeks after returning (watch for neuropsychiatric side effects).
  • Chloroquine (where sensitive): weekly dosing starting 1–2 weeks before, weekly during travel, and 4 weeks after return.

D. Vaccine Developments

  • RTS,S/AS01 (Mosquirix™):
    • WHO recommended in October 2021 for broad use in children in regions with moderate to high P. falciparum transmission.
    • Provides partial protection; efficacy wanes over time, booster doses needed.
  • R21/Matrix-M:
    • Shows promising Phase II/III results with higher efficacy; licensed in some African pilot programs (as of mid-2023).

7. Global Burden & Epidemiology

  • Estimated Cases (2023): ~247 million cases worldwide.
  • Estimated Deaths (2023): ~608,000 deaths, mostly children under 5 in sub-Saharan Africa.
  • High-Burden Countries: Nigeria, Democratic Republic of the Congo, Uganda, Mozambique, and Burkina Faso account for over half of the global malaria burden.
  • Seasonality: Transmission often peaks during or just after rainy seasons when mosquito breeding sites are plentiful.
  • Resistance Patterns:
    • Artemisinin resistance has emerged in parts of Southeast Asia (e.g., Cambodia, Thailand, Myanmar border regions).
    • Chloroquine resistance is widespread for P. falciparum and increasingly reported for P. vivax in certain areas of Oceania and South America.

8. Special Populations

  • Pregnant Women:
    • Higher risk of severe malaria, miscarriage, stillbirth, and low birth weight.
    • Intermittent preventive therapy in pregnancy (IPTp) with sulfadoxine–pyrimethamine is recommended in endemic areas.
  • Infants and Young Children:
    • Account for the majority of malaria mortality; limited immunity leads to rapid progression to severe disease.
  • Immunocompromised Patients (e.g., HIV Co-infection):
    • Higher risk of severe disease and treatment failures; require close monitoring.

9. Complications & Long-Term Sequelae

  • Neurological Impairment: Post-cerebral malaria survivors (especially children) may develop cognitive deficits, motor abnormalities, or behavioral issues.
  • Anemia & Splenomegaly: Chronic or repeated infections can cause splenic enlargement and chronic hemolytic anemia.
  • Renal Failure: Blackwater fever (intravascular hemolysis) can lead to acute tubular necrosis.
  • Respiratory Distress: Pulmonary edema may occur in severe P. falciparum.

10. Summary of Key Points

  • Malaria is caused by Plasmodium parasites transmitted by infected Anopheles mosquitoes.
  • Classic symptoms include cyclical fevers, chills, headache, myalgia, and gastrointestinal complaints such as nausea and vomiting.
  • Diagnosis relies primarily on microscopy, with RDTs and PCR serving as adjuncts.
  • Treatment varies by species and severity; ACTs are first-line for uncomplicated P. falciparum, while chloroquine + primaquine is used for P. vivax/ovale.
  • Preventive strategies—ITNs, IRS, chemoprophylaxis, and (where available) vaccination—are critical to reduce morbidity and mortality.
  • Global control efforts focus on reducing transmission, managing drug resistance, and expanding vaccine coverage.

Common Symptom Highlights

  • Nausea and Vomiting: Frequently reported, especially in high-parasitemia P. falciparum cases; can precede the onset of fever.
  • Diarrhea: Occurs in a subset of patients (more often children), may be mild to moderate.

By understanding the parasite life cycle, clinical features, diagnostic tools, and treatment/prevention modalities, healthcare providers can both manage individual cases and contribute to broader malaria control initiatives.

 

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